Simone Heber, currently postdoc in our lab, received a travel grant from the Boehringer-Ingelheim Fonds for a lab visit to Tamir Gonen’s lab at UCLA. The aim of this visit is to learn sample preparation as well as data collection and processing by microED.
Before we all participated in the congress “The complex life of RNA” in Heidelberg, we had a short satellite meeting. Apart from the great symposium, we had a very interesting meeting picked with exciting talks on diverse topics in RNA biology.
From March 3rd to 6th 2018 our RNA transport meeting took place in Düsseldorf-Kaiserswerth, where various experts from a broad field of RNA biology were representing the current state of the mRNA-localization field.
It was a very exciting congress with outstanding keynote lectures and 9 sessions packed with excellent talks. Of course, we also had lots of fun!
At the cellular level, learning and memory rely on the physical modification of synapses, which are ultimately encoded by transported mRNAs and protein synthesis “onsite”. In previous studies, Michael Kiebler and his team have shown that the RNA-binding protein Staufen2 is essential in conveying mRNAs to their destinations. Now, a new study carried out by the Kiebler group and colleagues shows that reduced levels of Staufen2 are associated with a specific impairment of memory. Using a genetic model in which Staufen2 can be conditionally and selectively suppressed in nerve cells in the rat forebrain, they observed that Staufen2 depletion affected nerve-cell morphology, synaptic function and impaired spatial, temporal and associative memory. The findings appear in the journal Genome Biology.
Stefan M. Berger, Iván Fernández-Lamo, Kai Schönig, Sandra M. Fernández Moya, Janina Ehses, Rico Schieweck, Stefano Clementi, Thomas Enkel, Sascha Grothe, Oliver von Bohlen und Halbach, Inmaculada Segura, José María Delgado-García, Agnès Gruart, Michael A. Kiebler and Dusan Bartsch.
Forebrain-specific, conditional silencing of Staufen2 alters synaptic plasticity, learning and memory in rats.
Genome Biology 2017 Nov 17;18(1):222. doi: 10.1186/s13059-017-1350-8.
The Niessing, Jansen and Feldbrügge labs published a review on recent advances in mRNA localization in different fungal species. The review appeared in WIREs RNA and puts a special focus on the comparison of membrane-free and membrane-bound RNA transport.
Niessing, Jansen, Pohlmann & Feldbrügge
mRNA transport in fungal top models
WIREs RNA DOI: 10.1002/wrna.1453 (2017)
You can find a wrap-up of this article on the website of [Advanced Science News].
This time our regular network meeting took place in the cozy town of Tübingen in the middle of Baden-Württemberg. It was a wonderful get-together and a great opportunity to discuss the latest scientific results in RNA localization.
Thanks to Ralf-Peter Jansen and Fulvia Bono for organizing this wonderful meeting!
Lab members of the FOR2333 network participated in the EMBO conference “RNA localisation and local translation” in Barga, Italy. The conference took place from July 23rd to 28th 2017 at the beautiful Il Ciocco Resort in Tuscany and was packed with great talks and exciting science. Thanks to the organisers for this wonderful meeting!
In particular in highly polarized cells like fungal hyphal early endosomes carry out two distinct functions. Firstly, during endocytosis they function as transport unit to deliver cargo towards the lysosome/vacuole. Secondly, the same early endosomes are used to deliver cargo like mRNAs, associated ribosomes as well as protein complexes over long distances to ensure efficient polar growth. Studying the evolutionarily conserved protein Did2 revealed that this ESCRT regulator is needed to coordinate these two seemingly independent functions. This has implications for the role of early endosomes in other trafficking processes like during neuronal mRNA transport.
Haag, C., Pohlmann, T., Feldbrügge, M.
The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking.
PLoS Genet 13(4): e1006734 (2017).
From March 13.-15. 2017 the FOR2333 team held a three-days methods workshop at the Advanced Training Centre of the EMBL Heidelberg.
Students of participating groups attended training sessions on “bioinformatics”, “professional preparation of scientific figures”, “introduction to structural biology”, and “high-resolution imaging and image processing”. The sessions consisted of lectures and hands-on training, and were
designed to support students and postdocs in their own project.
Read more ›
In this study, the Ephrussi lab shows that an atypical tropomyosin isoform is a direct (m)RNA binding protein that binds preferentially to the dimerizing oskar 3’ UTR and is a component of the transported oskar mRNPs within the female germ-line. In the absence of this tropomyosin isoform, Khc fails to get loaded onto oskar mRNA, which explains the reduced motility and ultimately the failure in oskar localization. This Tm1-I/C dependent recruitment is rather inefficient – only a small fraction of oskar mRNPs acquire Khc – but dynamic, enabling the posterior-ward transport of virtually all oskar mRNPs. Most importantly, however, the Tm1-I/C recruited Khc is inactive. Activation of the motor only commences in the oocyte during mid-oogenesis – possibly to prevent interference with the other transporter of oskar, cytoplasmic dynein – and requires the previously identified exon junction complex (EJC) and associated spliced oskar localization element (SOLE).
Gaspar, I., Sysoev, V., Komissarov, A. and Ephrussi, A. (2016)
An RNA-binding atypical tropomyosin recruits kinesin-1 dynamically to oskar mRNPs.
EMBO J. DOI 10.15252/embj.201696038